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This is due in part to the use of fluorescent resonance energy transfer (FRET), fluorophores were carefully selected such that, when the fluorophores are paired, activation of the donor fluorophore causes a highly efficient energy transfer to the acceptor fluorophore, producing a large emission from the acceptor fluorophore, this effect diminishes exponentially as the distance between the fluorophores increases

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Upon recognition, unfolding and transfer of the de-ubiquitinated target protein by the 19S regulatory cap into the interior of the cylindrical 20S proteasome core particle, protein degradation is facilitated by catalytic -subunits having nucleophilic N-terminal threonine (Thr1) residues

Similarly, drugs from the class of aromatase inhibitors (AIs), including letrozole, anastrozole, and exemestane, have also shown positive results in postmenopausal women (214)